Seeding calculator

Calculate the amount of cell suspension and medium you need for your cell harvest.

 

Seeding calculatior cell culture

 

Vc = Volume of cell suspension [mL]

r = growth rate [1/d] t = growth time [h]

ρ 0 = cel suspension density [cells/mL]

ρ t = planned harvest density [cells/cm2]

Vd = device volume [mL]

Nt = total harvest [cells/device]

 

    \[V_c = \frac{ \exp(r \cdot\frac{t}{ 24}\cdot ln(\frac{ \rho_t \cdot V_d}{ N_t})}{\rho_0}\]